OBJECTIVE： To provide reference for improving rational drug use in undergraduates from non-medical college. METHODS： By questionnaire survey， 9 non-medical colleges were collected according to college entrance examination admission batches and school types stratification sampling. The questionnaires were issued among 860 college students by encounter sampling. The survey included medication knowledge， medication behavior， personal basic information three aspects. Single factor analysis and Logistic regression analysis were conducted for their influential factors. RESULTS： A total of 860 questionnaires were sent out， and 812 valid questionnaires were collected with effective recovery rate of 94.4％. In respect of medication knowledge， 4.2％ of college students answered all of the five questions correctly， the correlation of correct rate with monthly disposable income was maximal （P＝0.007）； correct rate of the minority students with monthly disposable income more than 6 000 yuan was higher. In respect of medication behavior， 14.4％ of college students answered all of the seven questions correctly， the correlation of correct rate with whether the family had long-term （more than half a year） medication experience was maximal （P＝0.035）； the students whose family had no long-term （more than half a year） medication experience member and had medical personnel used drugs more standardly. CONCLUSIONS： The non-medical college students in Beijing lack of medication knowledge and have poor compliance； medication behavior is also unreasonable. Society， universities and families should strengthen the health education of students， and guide students to standardize the self-medicine therapy.