OBJECTIVE： To study the chemical constituents in the chloroform extract of Fructus Canarii. METHODS： The chloroform extract of Fructus Canarii was isolated and purified by MCI， Toyopearl HW-40F， reverse-phase C18， Sephadex LH-20 column. The structure of compounds was analyzed and identified by physicochemical properties and spectral data （mass spectrum， hydrogen spectrum and carbon spectrum）. RESULTS： Eight compounds were isolated from chloroform extract of Fructus Canarii， namely Dihydrophaseicacid-3′ -O-β-D-glucopyranoside（1）， 3，4-Dihydroxybenzoic acid（2）， 3-O-galloylquinic acid（3）， Gallic acid （4）， Ethyl gallate（5）， Scopoletin（6）， Dllagic acid-4-O-α-L-rhamnopyranoside（7） and Isocorilagin（8）. CONCLUSIONS： Compounds 1， 2 and 7 were firstly obtained from Fructus Canarii. The study lays foundation for quality evaluation of Fructus Canarii.