OBJECTIVE： To establish the method for the simultaneous determination of content of 6 active components as neochlorogenic acid， mulberroside A， chlorogenic acid， astragalin， sanggenon C and morusin in Morus alba， and to provide reference for improving quality control standard of M. alba. METHODS： HPLC method was adopted. The determination was performed on Agilent 5 TC-C18 with mobile phase consisted of acetonitrile-0.1％ formic acid （gradient elution） at the flow rate of 1 mL/min. The detection wavelength of 280 nm. RESULTS： The mass concentration linear range of neochlorogenic acid， mulberroside A， chlorogenic acid， astragalin， sanggenon C and morusin were 0.001 06-0.042 4， 0.001 67-0.066 8， 0.007 95-0.318， 0.001 65-0.066 0， 0.005 00-0.200 and 0.001 24-0.049 6 mg/mL， respectively （all r≥0.999 6）； the limits of quantitation were 0.11， 0.14， 0.81， 0.17， 0.45 and 0.12 μg/mL， respectively； the limits of detection were 0.04， 0.05， 0.41， 0.07， 0.18 and 0.04 μg/mL， respectively； RSDs of precision test were 0.26％， 0.31％， 0.24％， 0.27％， 0.36％ and 0.44％ （n＝6）， respectively； RSDs of stability test were 0.68％， 0.54％， 0.62％， 0.53％， 0.41％ and 0.73％（n＝6）， respectively； average method recovery rates were 99.1％， 98.8％， 98.8％， 98.4％， 98.5％ and 99.9％（RSDs were 0.5％-1.5％， n＝9）， respectively. CONCLUSIONS： The method is simple， accurate， and can be used for simultaneous determination of 6 active components in M. alba.