OBJECTIVE： To establish HPLC fingerprint of Miao medicine Fufukang spray. METHODS： The determination was performed on Thermo Hypersil GOLD C18 column with mobile phase consisted of acetonitrile-0.2％ glacial acetic acid （gradient elution） at the flow rate of 0.6 mL/min. The detection wavelength was set at 262 nm， and column temperature was 25 ℃. The sample size was 10 μL. Using 6′ -hydroxyl-justicidin B as reference control， HPLC chromatograms of 10 batches of samples were drawn. Similarity evaluation system software （2004 A edition） of TCM fingerprint was used to evaluate the similarity and determine common peaks of 10 batches of samples. The established method was used to determine the contents of 6′ -hydroxyl-justicidin B and justicidin B in 10 batches of samples. RESULTS： There were 12 common peaks in HPLC fingerprints of 10 batches of samples with similarity＞0.90. HPLC fingerprint showed a good consistency with the control fingerprint. The contents of 6′ -hydroxyl- justicidin B and justicidin B in 10 batches of samples were 1.64-5.84， 0.83-2.78 mg/100 mL， respectively. CONCLUSIONS： The established method is simple， accurate， reliable and suitable for quality evaluation of Fufukang spray.