OBJECTIVE： To establish a method for simultaneous determination of vitamin B1， dihydralazine sulfate， vitamin B6 and vitamin B5 in Compound Apocynum venetum tabletsⅠ. METHODS： HPLC method was used for content determination of 8 batches of samples from 2 enterprises. The determination was performed on Waters XBridge C18 column with mobile phase consisted of acetonitrile-0.2％ phosphoric acid （gradient elution） at the flow rate of 0.5 mL/min.  The detection wavelengths were set at 285 nm （vitamin B1， dihydralazine sulfate， vitamin B6） and 210 nm （vitamin B5）. The column temperature was 30 ℃， and sample size was 10 μL. RESULTS： The linear ranges of vitamin B1， dihydralazine sulfate， vitamin B6 and vitamin B5 were 41.66-208.3 μg/mL （r＝0.999 9）， 127.0-635.0 μg/mL （r＝0.999 9）， 40.74-203.7 μg/mL （r＝0.999 9） and 22.72-113.6 μg/mL （r＝0.999 9）， respectively. The limits of quantitation were 0.267， 0.274， 0.062， 0.124 μg/mL， respectively. The limits of detection were 0.095， 0.088， 0.021， 0.039 μg/mL， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％ （n＝6）. The average recoveries were 98.70％ （RSD＝0.69％， n＝9）， 99.74％ （RSD＝1.2％， n＝9）， 99.89％ （RSD＝1.0％， n＝9）， 99.25％ （RSD＝1.2％， n＝9）， respectively. Among 8 batches of samples， the contents of vitamin B1， dihydralazine sulfate， vitamin B6 and vitamin B5 were 91.44％-98.74％， 36.68％-95.23％， 92.42％-97.90％， 91.36％-99.48％ of labelled amount， respectively. The contents of dihydralazine sulfate were significantly different and differed by 2.6 times between the lowest and the highest threshold value. CONCLUSIONS： The method is accurate， reliable， simple and can be used for simultaneous determination of the above 4 components in Compound A. venetum tabletsⅠ.