OBJECTIVE： To provide reference for further promoting rational use of antibiotics in hospitals and curbing bacterial drug resistance. METHODS： Retrospective investigation and comparative analysis were made on the clinical application of antibiotics， the inspection of microbial specimens before use of antibiotics， the detection of multidrug-resistant bacteria， the occurrence of nosocomial infection before and after multi-sectoral participation in the prevention and control of multi-resistant bacteria infection in our hospital （from Jul. 2015 to Jun. 2016 as before intervention and from Oct. 2016 to Sept. 2017 as after intervention） in order to evaluate the intervention effect of this work. RESULTS： After intervention， the utilization rate of antibiotics， antibiotics use intensity and rate of combined use decreased from 64.15％， 48.86 DDDs/hundred person per day， 35.87％ to 57.67％， 36.58 DDDs/hundred person per day， 11.47％， respectively （P＜0.05）. Both the de-escalation use rate of special grade antibiotics and the rate of antibiotics choice based on the drug sensitivity test increased from 12.45％ and 48.28％ to 56.63％ and 77.89％ （P＜0.001）. Microbiological specimen inspection rate， total specimen inspection rate before using non-restricted use， restricted use and special use antibiotics and the sterile specimen inspection rate before using antibiotics increased from 23.58％， 43.15％， 71.76％， 36.37％ and 20.82％ to 40.61％， 58.43％， 95.77％， 51.33％ and 38.27％， respectively （P＜0.05 or P＜0.01）. The detection rate of MRSA， CRE and MDR-PA decreased from 4.43％， 2.80％， 1.99％ to 1.36％， 1.26％， 0.80％， respectively （P＜0.05 or P＜0.01）， and the detection rates of multi-resistant bacteria decreased from 16.21％ to 10.68％ （P＜0.05）. Meanwhile， the incidence of nosocomial infection decreased from 1.41％ to 1.08％ （P＜0.05）， and there was no outbreak of nosocomial infection. CONCLUSIONS： The intervention effect of multi-department participation in prevention and control of multi-resistant bacterial infection is significant， which helps to promote rational use of antibiotics and curb bacterial drug resistance， and it is necessary to continue to operate as a long-term management mechanism.