OBJECTIVE： To establish the method for simultaneous determination of the content of berberine hydrochloride，baicalin and osthole in Jinchan zhiyang capsules. METHODS： HPLC method was adopted. The determination was performed in Hypersil BDS C18 column with mobile phase consisted of methanol-acetonitrile-0.1％ phosphoric acid-three triethylamine （50 ∶ 30 ∶ 19 ∶ 1，V/V/V/V） at the flow rate of 1.0 mL/min. The detection wavelengths were set at 265 nm （berberine hydrochloride）， 280 nm （baicalin） and 322 nm （osthole）. The column temperature was set at 30 ℃， and sample size was 10 μL. RESULTS： The linear range of berberine hydrochloride， baicalin and osthole were 80-800 ng（r＝0.999 8）， 60-600 ng（r＝0.999 9）， 60-600 ng（r＝0.999 6）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The limits of quantitation were 80，60，60 ng， respectively， and the limits of detection were 24，20，20 ng， respectively. The recovery rates were 97.4％-98.3％（RSD＝0.33％，n＝6）， 98.4％-99.6％（RSD＝0.42％，n＝6） and 96.9％-99.0％（RSD＝0.92％，n＝6）， respectively. RSDs of durability tests were all lower than 1.2％. CONCLUSIONS： The method is simple， accurate， precise， stable， reproducible and durable. It can be used for simultaneous determination of berberine hydrochloride，baicalin and osthole in Jinchan zhiyang capsules.