OBJECTIVE： To study the effects of scutellarin on the proliferation of cardiac fibroblasts （CFs） in neonate rats induced by angiotensin Ⅱ （ANGⅡ） and signal pathway of extracellular regulated protein kinase （ERK1/2） and p38 mitogen activated protein kinase （p38 MAPK）. METHODS： CFs of neonatal rat were isolated and cultured in vitro， and then divided into blank group （blank culture medium）， Ang Ⅱ group （10－7 μmol/L），50 μmol/L scutellarin group and Ang Ⅱ（10－7 μmol/L）+5， 10， 20， 50 μmol/L scutellarin groups. After cultured for 48 h， CCK-8 assay was used to detect the proliferation ability of cells. Other cells were selected and divided into blank group （blank culture medium）， Ang Ⅱ group （10－7 μmol/L） and Ang Ⅱ+5， 10， 20， 50 μmol/L scutellarin groups. After cultured for 48 h， mRNA expression of ColⅠ， Col Ⅲ and α-SMA in cells and hydroxyproline （HYP） content in cell culture fluid were detected； phosphorylation levels of ERK1/2 and p38 MAPK in cells were also detected. RESULTS： 5， 10， 20， 50 μmol/L scutellarin could significantly inhibit the proliferation of CFs induced by Ang Ⅱ （P＜0.05）， and decreased mRNA expression of ColⅠ， Col Ⅲ and α-SMA in CFs induced Ang Ⅱ （P＜0.05）. 5， 10， 20， 50 μmol/L scutellarin could significantly inhibit the increase of HYP content and the phosphorylation of ERK1/2 and p38 MAPK after induced by Ang Ⅱ （P＜0.05）， in dose-dependent manner. CONCLUSIONS： Scutellarin inhibits the proliferation of CFs induced by Ang Ⅱ， the mechanism of which may be associated with reduction of ERK1/2 and p38 MAPK phosphorylation.