OBJECTIVE: To optimize the extraction technology for flavonoids in leaves and seeds of Hippophae rhamnoides. METHODS: Using the total extraction rate of 6 flavonoid aglycones (catechins, rutin, myricetin, quercetin, kaempferol, isorhamnetin) as index, ethanol volume fraction, extraction time, extraction times, material-lipid ratio as investigation indexes, L9(34) orthogonal test was designed to optimize the extraction technology of flavonoids in leaves and seeds of H. rhamnoides, and verification test was carried out. RESULTS: The optimum extraction technology for flavonoids in leaves of H. rhamnoides was ethanol volume fraction of 70%, extracting for 3 times with material-lipid ratio of 1 ∶ 16, and 2.0 h each time; and that of seeds was ethanol volume fraction of 50%, extracting for 3 times with material-lipid ratio of 1 ∶ 24, and 1.5 h each time. In verification test, the total extraction rate of 6 flavonoid aglycones was 56.4 mg/g in the leaves (RSD=1.4%, n=3) and 15.4 mg/g in the seeds (RSD=3.4%, n=3). CONCLUSIONS: Optimized extraction technology is simple, stable, feasible, and can be used for extracting the flavonoids in leaves and seeds of H. rhamnoides.
关键词
沙棘叶沙棘籽正交试验提取工艺黄酮苷元
Keywords
Leaves of Hippophae rhamnoidesSeeds of Hippophae rhamnoidesOrthogonal testExtraction technologyFlavonoid aglycone