OBJECTIVE： To establish HPLC fingerprints of Potentilla discolor， and to conduct authenticity identification. METHODS： HPLC method was adopted. The determination was performed on InertSustain C18 column with mobile phase consisted of 0.1％ formic acid solution-acetonitrile （gradient elution） at the flow rate of 1.0 mL/min， detection wavelength of 360 nm， column temperature of 30 ℃， sample size of 10 μL. Using rutin as reference， HPLC chromatograms of 19 batches of P. discolor and 2 batches of P. chinesis were determined. TCM Fingerprint Similarity Evaluation System （2004） was used for similarity evaluation of 21 batches of samples， and common peak identification of 19 batches of P. discolor. SPSS 21.0 statisticl software was used for main component analysis and cluster analysis. RESULTS： There were 18 common peaks in HPLC fingerprints of 19 batches of P. discolor， the similarity was higher than 0.9. HPLC chromatogram was in good agreement with control fingerprint. The similarity of 2 batches of P. chinesis was lower than 0.7. The 21 batches of medicinal materials could be grouped into 2 categories， 2 batches of P. chinesis could be grouped into a category， 19 batches of P. discolor could be grouped into a category. P. discolor could be grouped into 4 categories. Rutin and quercitrin were main ingredients in 19 batches of P. discolor. CONCLUSIONS： Established fingerprint can provide reference for authenticity identification and quality evaluation of P. discolor.