OBJECTIVE： To prepare borneol-modified colchicine ethosome， and to evaluate its in vitro transdermal permeation. METHODS： Borneol-modified colchicine ethosome was prepared with ultrasonic injection-probe ultrasonic method， and its ethosome was characterized. The content and moditication rate of borneol in borneol-modified colchicine ethosome was determined by GC； content and encapsulation rate of colchicine was determined by HPLC. Accumulative permeability and permeation rate were investigated for colchicine ethanol solution， colchicine-borneol-ethanol solution， colchicine ethosome without borneol-modification， borneol- modified colchicine ethosome after diffused for 1， 3， 6， 8， 12， 16， 24， 48 h. RESULTS： The average particle size of borneol- medified colchicine ethosome was about （110.4±5.1） nm， polydispersity index was 0.110±0.030， Zeta potential was （2.33±0.20） mV， prepared ethosome is approximately spherical in shape and multilaminar vesicles in structure. Encapsulation rate of colchicine was 56.12％， and modification rate was 9.85％. The in vitro diffusion tests showed that after diffused for 48 h， accumulative permeability per unit area of borneol-modified colchicine ethosome was 103.52 μg/cm2； permeation rate was 1.26 times as colchicine ethosome without borneol-modification， 1.77 times as colchicine-borneol-ethanol solution and 5.14 times as colchicine ethanol solution. CONCLUSIONS： Prepared borneol-modified colchicine ethosome has small particle size， narrow particle size distribution， high modification rate and good transdermal permeation.