OBJECTIVE： To study the effects of Astragali radix injection combined with entecavir on serum inflammatory cytokines and liver function of model mice with hepatitis B. METHODS： 60 mice were randomly divided into normal control group （normal saline）， model group （normal saline）， Astragali radix injection group （0.5 mL/10 g）， entecavir group （45 μg/kg） and combination group （0.5 mL/10 g Astragali radix injection+45 μg/kg entecavir）， 12 in each group. Except for normal control group， mice in other 4 groups were induced for hepatitis B models. After modeling， all mice were intragastrically administrated once a day， for 4 weeks. After administration， tumor necrosis factor α （TNF-α）， interferon γ （IFN-γ）， interleukin 8 （IL-8）， aspartate aminotransferase （AST）， alanine aminotransferase （ALT）， total bilirubin （TBIL） levels in serum were detected， and the pathological changes in liver tissue in each group were observed. RESULTS： Compared with normal control group， TNF-α， IFN-γ， IL-8， AST， ALT， TBIL levels in serum of mice in model group were significantly increased （P＜0.05）； and liver cells were diffuse and severe steatosis. Compared with model group， TNF-α， IFN-γ， IL-8， AST， ALT， TBIL levels in serum of mice in each administration group were significantly decreased （P＜0.05）， pathological changes in liver tissue were improved to varying degrees， and the index improvement degree in combination group was superior to Astragali radix injection group and entecavir group （P＜0.05）. CONCLUSIONS： Astragali radix injection combined with entecavir helps to down-regulate the expressions of inflammatory cytokines of model mice with hepatitis B， and improve the liver function. The combination use has better effect than single use.