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目的:研究三白草药材不同部位中微量元素的差异。方法:采用微波消解法处理药材;采用电感耦合等离子体质谱法测定药材不同药用部位中25种微量元素含量:射频功率为1 200 W,冷却气流量为14 L/min,辅助气流量为1.0 L/min,雾化气流量为0.9 L/min,碰撞气体为氦,碰撞气流速为4.8 mL/min,采样锥孔径为1.1 mm,截取锥孔径为0.9 mm,超级截取锥孔径为1.0 mm,积分时间为1 s,延迟时间为40 s,扫描方式为单点跳峰,测定重复3次;采用SIMCA-P 13.0统计软件对数据进行主成分分析和偏最小二乘判别分析。结果:锂(Li)、铍(Be)、硼(B)、镁(Mg)、铝(Al)、钛(Ti)、钒(V)、铬(Cr)、锰(Mn)、铁(Fe)、钴(Co)、镍(Ni)、铜(Cu)、锌(Zn)、镓(Ga)、砷(As)、钴(Sr)、镉(Cd)、锡(Sn)、锑(Sb)、钡(Ba)、汞(Hg)、铊(Ti)、铅(Pb)、铋(Bi)检测质量浓度线性范围分别为0.05~5.0 μg/L(r=0.999 9)、0.05~5.0 μg/L(r=0.999 8)、0.10~10.0 μg/L(r=0.999 5)、1.00~100.0 μg/L(r=0.998 5)、1.00~100.0 μg/L(r=0.999 3)、0.10~10.0 μg/L(r=0.999 9)、0.05~5.0 μg/L(r=0.999 7)、0.05~5.0 μg/L(r=0.999 5)、0.10~10.0 μg/L(r=0.999 8)、1.00~100.0 μg/L(r=0.999 4)、0.05~5.0 μg/L(r=0.999 9)、0.05~5.0 μg/L(r=0.999 7)、0.05~5.0 μg/L(r=0.999 5)、0.05~5.0 μg/L(r=0.999 8)、0.05~5.0 μg/L(r=0.999 6)、0.05~5.0 μg/L(r=0.999 8)、0.05~5.0 μg/L(r=0.999 9)、0.05~5.0 μg/L(r=0.999 3)、0.05~5.0 μg/L(r=0.999 4)、0.05~5.0 μg/L(r=0.999 8)、0.10~10.0 μg/L(r=0.999 6)、0.05~5.0 μg/L(r=0.999 6)、0.05~5.0 μg/L(r=0.999 6)、0.05~5.0 μg/L(r=0.999 7)、0.05~5.0 μg/L(r=0.999 4);检测限≤13.2 ng/L;精密度、稳定性、重复性试验的RSD<5.12%;加样回收率为90.18%~123.01%(RSD为2.05%~5.79%,n=6)。Sb、Sn、Mn、Cd、Ti、Ba、Co、As、Be、Ga、Ni在药材样品地上部分含量较根茎中高,而Cr在根茎中含量较地上部分高。结论:三白草药材地上部分和根茎中Mg、Fe、Al等含量较为丰富,不同药用部位中所含微量元素含量差异较显著。
OBJECTIVE: To analyze the differences of inorganic elements in different parts of Saururus chinensis. METHODS: The samples were treated with microwave digestion. The contents of 25 inorganic elements were determined by inductively coupled plasma mass spectrometry (ICP-MS) method: RF power of 1 200 W, cooling gas flow of 14 L/min, auxiliary gas flow of 1.0 L/min, atomizing air flow of 0.9 L/min, He as collision gas with flow rate of 4.8 mL/min, sampling cone aperture of 1.1 mm, skimmer cone aperture of 0.9 mm, super skimmer cone aperture of 1.0 mm, integral time of 1 s, delay time of 40 s, single hop as scanning mode, repeated for 3 times. The data of principal component analysis and partial least squares discriminant analysis were analyzed with the SIMCA-P 13.0 statistical software. RESULTS: The linear ranges of lithium (Li), beryllium (Be), boron (B), magnesium (Mg), aluminum (Al), titanium (Ti), vanadium (V), chromium (Cr), manganese (Mn), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), gallium (Ga), arsenic (As), cobalt (Sr), cadmium (Cd), tin (Sn), antimony (Sb), barium (Ba), mercury (Hg), thallium (Ti), lead (Pb) and bismuth (Bi) were 0.05-5.0 μg/L(r=0.999 9), 0.05-5.0 μg/L(r=0.999 8), 0.1-10.0 μg/L(r=0.999 5), 1.0-100.0 μg/L(r=0.999 5),1.00-100.0 μg/L(r=0.999 3), 0.10-10.0 μg/L(r=0.999 9), 0.05-5.0 μg/L(r=0.999 7),0.05-5.0 μg/L(r=0.999 5), 0.10-10.0 μg/L(r=0.999 8), 1.00-100.0 μg/L(r=0.999 4),0.05-5.0 μg/L(r=0.999 9), 0.05-5.0 μg/L(r=0.999 7),0.05-5.0 μg/L(r=0.999 5),0.05-5.0 μg/L(r=0.999 8),0.05-5.0 μg/L(r=0.999 6), 0.05-5.0 μg/L(r=0.999 8),0.05-5.0 μg/L(r=0.999 9),0.05-5.0 μg/L(r=0.999 3),0.05-5.0 μg/L(r=0.999 4),0.05-5.0 μg/L(r=0.999 8),0.1-10.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 6),0.05-5.0 μg/L(r=0.999 7), 0.05-5.0 μg/L(r=0.999 4), respectively. The detection limits were no higher than 13.2 ng/L. RSDs of precision, stability and reproducibility tests were all lower than 5.12%.The recoveries ranged 90.18%-123.01% (RSD were 2.05%-5.79%,n=6). The contents of Sb, Sn, Mn, Cd, Ti, Ba, Co, As, Be,Ga,Ni were higher in aerial part than in rhizome part, while the content of Cr was higher in rhizome part than in aerial part. CONCLUSIONS: Mg, Fe and Al are abundant in aerial part and rhizome part of S. chinensis. The contents of inorganic elements are significantly different in different parts of S. chinensis.
三白草电感耦合等离子体质谱法微量元素主成分分析偏最小二乘判别分析
Saururus chinensisICP-MSTrace elementsPrincipal component analysisPLS-DA
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