OBJECTIVE： To establish a method for simultaneous determination of eugenol and thymol in mummification pastes. METHODS： HPLC method was adopted. The determination was performed on Wonda Cract C18 column with mobile phase consisted of methanol-0.2％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was 274 nm， and column temperature was set at 25 ℃. The sample size was 10 μL. RESULTS： The linear ranges of eugenol and thymol were 0.02-0.19 mg/mL （r＝0.999 7） and 0.03-0.30 mg/mL （r＝0.999 6）. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were 95.85％-99.84％（RSD＝1.11％，n＝9） and 95.81％-100.91％（RSD＝1.59％，n＝9）. CONCLUSIONS： The method is simple and accurate， and can be applied to simultaneous determination of eugenol and thymol in mummification pastes.