OBJECTIVE： To establish a method for simultaneous determination of 7 constituents in Wikstroemia indica， and to conduct principal component analysis and cluster analysis. METHODS： HPLC method was adopted. The determination was performed on Diamonsil Platisil ODS column with mobile phase consisted of acetonitrile-0.15％ triethylamine solution （pH adjusted to 6.0 with phosphoric acid， gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm， and column temperature was 40 ℃. The sample size was 10 μL. The principal component analysis and cluster analysis were conducted for the results of content determination by SPSS 22.0 statistical software. RESULTS： The linear ranges were 2.688-53.76 μg/mL for naringin （r＝0.999 8）， 5.052-101.00 μg/mL for myricetin （r＝0.999 9）， 2.052-41.04 μg/mL for arctiin （r＝0.999 9）， 2.108-42.16 μg/mL（r＝0.999 9）， 5.112-102.20 μg/mL （r＝0.999 9）， 0.820-16.42 μg/mL （r＝0.999 9）， 2.070-41.40 μg/mL（r＝0.999 9）， respectively. The limits of quantitation were no higher than 1.072 0 μg/mL， the limits of detection were no higher than 0.331 8 μg/mL. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were 97.8％-102.5％（RSD＝1.8％，n＝6），97.2％-102.0％（RSD＝2.0％，n＝6），95.2％-100.1％（RSD＝1.7％，n＝6），95.2％-99.3％（RSD＝1.6％，n＝6）， 97.0％-100.8％（RSD＝1.3％，n＝6）， 95.5％-98.6％（RSD＝1.1％，n＝6）， 95.0％-99.3％（RSD＝1.8％，n＝6）， respectively. Three main components were belong to the samples of 10 batches of medicinal materials. The samples of medicinal materials from 10 producing area could be divided into 2 categories. The quality of W. indica from Qingyuan Guangdong and Guiyang Guizhou were better than others.  CONCLUSIONS： The method is simple， precise， stable and reproducible， and it can be used for simultaneous determination of 7 constituents in medicinal material. The quality of W. indica from different regions are quite different.