OBJECTIVE： To provide reference for rational use of vancomycin. METHODS： The medical records of 299 patients receiving vancomycin in our hospital during Jan. 2015-Jun. 2016 were collected and analyzed statistically in respects of drug use， medication rationality， etiological test results， blood concentration of vancomycin， therapeutic efficacy， adverse drug reaction， etc. RESULTS： Vancomycin was mostly used as therapy drug in our hospital， mainly empiric treatment （90.30％）； detection rate of samples before medication was 61.82％， and specific pathogens were detected in 52 cases （28.42％）. There were 69 cases of irrational drug use （23.08％）， which were unsuitable drug concentration. Monitoring rate of vancomycin concentration was 43.33％； trough concentration remained at 10-20 mg/L， accounting for 40.00％. Response rate was 79.39％. The incidence of acute kidney injury was 5.41％ after treatment. CONCLUSIONS： The examination rate before medication and blood concentration monitoring rate of vancomycin are in low level in our hospital. The patients with effective trough blood concentration accounts for low proportion. Clinical application management of vancomycin should be strengthened to promote individual medication.