OBJECTIVE： To study the effects of ginsenoside CK combined with 5-fluorouracil （5-FU） on the proliferation， apoptosis and epithelial mesenchymal transition （EMT） of human pancreatic cancer PANC-1 cells. METHODS： PANC-1 cells of logarithmic growth phase were randomly divided into blank control group， ginsenoside CK group （30 mg/L）， 5-FU group （25   mg/L） and combination group （ginsenoside CK 30 mg/L+5-FU 25 mg/L）. MTT method was used to detect the cell proliferation inhibition rate in each group after 24， 48， 72 h； flow cytometry was used to detect the cell apoptosis rate after 48 h； enzyme-linked immunosorbent assay was used to detect the fibronectin expression in cells after 24， 48， 72， 96 h； and Western blot was used to detect the expressions of vimentin， N-cadherin， E-cadherin， protein kinase （Akt） and phosphorylated Akt （p-Akt） protein in cells after 48 h. RESULTS： Compared with blank control group， the cell proliferation inhibition rate， early and late apoptotic rates， protein expression level of E-cadherin in ginsenoside CK group， 5-FU group and combination group were obviously increased （P＜0.05）， while the protein expression levels of fibronectin， vimentin， N-cadherin， and p-Akt/Akt levels were obviously decreased （P＜0.05）； the effects of above-mentioned indexes in combination group were superior to ginsenoside CK group and 5-FU group （P＜0.05）. CONCLUSIONS： Both ginsenoside CK and 5-FU can inhibit the proliferation of PANC-1 cells， induce their apoptosis and inhibit EMT， which may be associated with inhibiting phosphatidylinositol 3-kinase/Akt pathway. In addition， the combination of ginsenoside CK and 5-FU can produce a better effect.