OBJECTIVE： To establish a method for the concentration determination of voriconazole in human plasma. METHODS： Plasma samples were precipitated with acetonitrile. Using ketoconazole as internal standard， HPLC method was adopted. The determination was performed on Dionex U-3000 Dimonsil C18 column with mobile phase consisted of triethylamine-glacial acetic acid-water mixed solution （1 ∶ 1 ∶ 98，V/V/V， pH was about 4.0）-acetonitrile （40 ∶ 60，V/V） at the flow rate of 1.0 mL/min. The detection wavelength was set at 255 nm. The column temperature was 40 ℃， and sample size was 20 μL. RESULTS： The linear range of voriconazole was 0.2-20.0 μg/mL. The limits of quantification was 0.2 μg/mL， and the minimum detection limit was 0.03 μg/mL. RSDs of inter-day and intra-day were lower than 10％. The method recoveries were 92.06％-106.26％（RSD＜5％，n＝5）， and extraction recoveries were 75.62％-90.59％（RSD＜5％，n＝5）. The plasma concentration of voriconazole in 10 children ranged 0.22-4.90 μg/mL （n＝10）. CONCLUSIONS： The method is simple， rapid， specific and can be used for drug monitoring of voriconazole.