OBJECTIVE： To prepare Formononetin （FMN） inclusion compound liposome and evaluate its quality. METHODS： FMN inclusion compound liposome was prepared by film dispersion method. The morphology， particle size， Zeta potential， encapsulation efficiency and in vitro release properties were studied. RESULTS： The particle size， Zeta potential and encapsulation efficiency of prepared FMN inclusion compound liposome were （255.34±12.87） nm， （25.32±3.51） mV， （81.63±0.79）％， respectively （n＝3）. The 24 h accumulative release rate of prepared FMN inclusion compound liposome was 56.12％. CONCLUSIONS： FMN inclusion compound liposome with good sustained-release effect is prepared successfully and in line with related quality standard.