OBJECTIVE： To establish HPLC fingerprint of Zhenqi fuzheng granules. METHODS： HPLC-ELSD method was adopted. The determination was performed on Agilent Zorbax Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 1.0 mL/min with column temperature of 40 ℃. The detector evaporation temperature was 50 ℃， and sample size was 10 μL. Using specnuezhenide as reference， HPLC chromatograms of 7 batches of Zhenqi fuzheng granules were determined. The identification and similarity evaluation of common peaks were conducted by using the TCM Chromatographic Fingerprint Similarity Evaluation System （2004 A edition）. RESULTS： There were 13 common peaks in HPLC chromatograms of 7 batches of samples. After validated， the similarity of 3 batches of samples in HPLC chromatograms of 7 batches of samples were higher than 0.9， which were in good agreement with control fingerprints. The similarity of 4 batches of samples were ＜0.9，which were poorly same to control fingerprint. CONCLUSIONS： Established fingerprint can provide reference for quality evaluation of Zhenqi fuzheng granules.