OBJECTIVE： To investigate the effects and safety of gamma globulin combined with phototherapy on serum prealbumin （PAB） and total bile acid （TBA） of ABO hemolytic children. METHODS： A total of 90 ABO hemolytic children in our hospital during Feb. 2014-Sept. 2016 were selected as research objects and divided into observation group and control group according to random number table， with 45 cases in each group. Both groups were given routine active correction of hypoxia and pretreatment for possible hypoglycemia and hypothermia. Control group was additionally given phototherapy with wavelength of 425-475 nm. Observation group was additionally given Human immunoglobulin for intravenous injection （pH4） 1.0g/kg was added to normal saline 10 mL， ivgtt， on the ?basis of control group and treated for 1 d. The time of jaundice regression， phototherapy duration and hospitalization time were compared between 2 groups， and the levels of PAB and TBA were compared before and after treatment. The occurrence of ADR was recorded in 2 groups. RESULTS： The time of jaundice regression， phototherapy duration and hospitalization time in observation group were significantly shorter than control group， with statistical significance （P＜0.05）. Before treatment， there was no statistical significance in the levels of PAB or TBA between 2 groups （P＞0.05）. After treatment， the level of PAB was increased significantly in 2 groups， while the level of TBA was decreased significantly； the observation group was significantly better than the control group， with statistical significance （P＜0.05）. There was no statistical significance in the incidence of ADR between 2 groups （P＞0.05）. CONCLUSIONS： Gamma globulin combined with phototherapy can significantly shorten the time of clinical symptom improvement， increase serum level of PAB whlie decrease serum level of TBA in ABO hemolytic children， with good safety.