OBJECTIVE： To study the chemical constituents of 70％ ethanol extract of Breynia fruticosa. METHODS： The 70％ ethanol extract of B. fruticosa were isolated and purified by D101 macroporous resin， silica gel column chromatography， RP-silica gel column chromatography， Sephadex LH-20 gel column chromatography， preparative HPLC. The structure of compound was analyzed and identified according to physicochemical characters and spectral data. RESULTS： 10 compounds were separated from 70％ ethanol extract of B. fruticosa， such as Luteolin （1），Quercetin （2），Kaempferol （3），Tiliroside （4），（+）-Lyoniresinol （5）， （+）-Isolariciresinol （6），（+）-Nortrachelogenin （7），（+）-Syringaresinol （8），Icariol A2（9）， 5，5′ -Dimethoxy-7-oxolariciresinol （10）， respectively. CONCLUSIONS： Ten compounds are isolated from this plant for the first time. The study play the found for quality evaluation of 70％ ethanol extract of B. fruticosa.