OBJECTIVE： To establish a method for simultaneous determination of ibuprofen and indomethacin concentration in human urine. METHODS： The urine samples were precipitated by acetonitrile. HPLC method was adopted. The determination was performed on Discovery C18 column with mobile phase consisted of acetonitrile-20 mmol/L ammonium acetate solution （85 ∶ 15，V/V， pH value adjusted to 3.5 with glacial acetic acid） at the flow rate of 1.0 mL/min. UV detection wavelength was set at 220 nm. The column temperature was room temperature， and sample size was 80 μL. RESULTS： The linear range of ibuprofen and indomethacin were both 0.1-50.0 μg/mL （r＝0.999 6， 0.999 5，n＝3）. The limits of quantitation were both 0.1 μg/mL， and the limits of detection were both 0.03 μg/mL. RSDs of inter-day and intra-day were all lower than 10％ （n＝5）， and accuracy ranged 94.7％-97.2％. The extraction recoveries of ibuprofen and indomethacin were 89.5％-91.8％ and 90.2％-92.4％ （all RSDs＜10％，n＝15）， respectively. CONCLUSIONS： The method is simple and rapid with high selectivity， sensitivity and accuracy. It is suitable for simultaneous determination of ibuprofen and indomethacin concentration in human urine.