OBJECTIVE： To develop a method for the simultaneous determination of 5 components in Shuganning injections. METHODS： HPLC method was adopted. The determination was performed on Symmetry® C18 column with mobile phase consisted of methanol-0.4％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavlengths were set at 238 nm （geniposide， baicalin） and 327 nm （chlorogenic acid， baicalein， scutellarin）. The column temperature was 30 ℃ and the sample size was 10 μL. RESULTS： The linear ranges were 0.406 2-26.0 μg/mL for chlorogenic acid （r＝0.999 9）， 2.500 0-160.0 μg/mL for geniposide （r＝0.999 9）， 6.562 0-420.0 μg/mL for baicalin （r＝0.999 9）， 0.312 5-20.0 μg/mL for baicalein （r＝0.999 6）， 0.585 9-37.5 μg/mL for scutellarin （r＝0.999 8）. The limits of quantify were no higher than 31.20 ng，limits of detection were no higher than 15.60 ng. RSDs of precision， stability and reproducibility tests were lower than 2.0％；the recoveries were 97.72％-101.10％（RSD＝1.21％，n＝6），97.67％-102.40％（RSD＝1.87％，n＝6），97.64％-101.10％（RSD＝1.31％，n＝6）， 96.45％-100.10％（RSD＝1.47％，n＝6）， 96.16％-101.10％ （RSD＝1.69％，n＝6）， respectively. CONCLUSIONS： The method is simple， precise， stable and reproducible， and can be used for simultaneous determination of 5 components in Shuganning injection.