OBJECTIVE： To establish a method for simultaneous determination of 6 components in Niuhuang ninggong tablets. METHODS： HPLC method was adopted. The determination was performed on TC-C18 column with mobile phase consisted of methanol-0.05％ phosphate acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 280 nm， and the column temperature was 25 ℃. The sample size was 10 μL. RESULTS： The linear ranges of glycyrrhizin， berberine hydrochloride， baicalin， baicalin， emodin and chrysophanol were 3.2-320 μg/mL（r＝0.999 9）， 8.8-880 μg/mL（r＝0.999 8）， 5.6-560 μg/mL （r＝0.999 5），2.0-200 μg/mL（r＝0.999 9）， 4.4-440 μg/mL （r＝0.999 9），2.0-200 μg/mL（r＝0.999 7）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 6.0％. The recoveries were 96.34％-97.25％（RSD＝0.33％，n＝6）， 96.12％-98.06％（RSD＝0.82％，n＝6）， 96.36％-99.09％（RSD＝1.02％，n＝6），95.84％-97.32％（RSD＝0.65％，n＝6）， 95.83％-97.92％（RSD＝0.88％，n＝6）， 98.60％-99.65％（RSD＝0.42％，n＝6）， respectively. CONCLUSIONS： The method is simple， accurate， stable and reproducible， and can be used for content determination of 6 components in Niuhuang ninggong tablets.