OBJECTIVE： To investigate the effects of clostridium butyricum combined with antibiotics on inflammatory factors and gastrointestinal function of patients with acute appendicitis after laparoscopic surgery. METHODS： A total of 102 patients with acute appendicitis undergoing laparoscopic surgery in our hospital during Jan. 2014-Jan. 2015 were divided into observation group and control group according to odd-even admission number， with 51 cases in each group. Control group was given Cefuroxime sodium for injection， Metronidazole injection and Cefixime dispersible tablets for anti-infective therapy after surgery. Observation group was additionally given Clostridium butyricum capsule 1 260 mg， tid， for consecutive 3 d， on the basis of control group. The levels of serum inflammatory factors （TNF-α， IL-6）， gastrin （GAS） and motilin （MTL） were compared between 2 groups before surgery and 3 d after surgery. The time of gastrointestinal function recovery （the time of bowel sounds， the time of first flatus， the time of postoperative first defecation） and the occurrence of compliance were recorded in 2 groups. RESULTS： Before surgery， there was no statistical significance in the levels of serum inflammatory and GAS， MTL factors between 2 groups （P＞0.05）. 3 d after surgery， the levels of TNF-α， IL-6 and CRP in 2 groups were decreased significantly， while the levels of GAS and MTL were increased significantly； above indexes of observation group were significantly better than those of control group， with statistical significance （P＜0.05）. The time of bowel sounds， the time of first flatus and the time of postoperative first defecation in observation group were significantly shorter than control group， with statistical significance （P＜0.05）. The incidence of complications in observation group was 3.92％， which was significantly lower than 15.69％ of control group（P＜0.05）. CONCLUSIONS： Clostridium butyricum combined with antibiotics can effectively relieve inflammatory state of patients with acute appendicitis after laparoscopic surgery， promote gastrointestinal function recovery， and reduce the occurrence of complications.