OBJECTIVE： To establish the method for simultaneous determination of epiberberine， jateorhizine hydrochloride， coptisine， palmatine hydrochloride and berberine hydrochloride in Lianshen tonglin tablets. METHODS： HPLC method was adopted. The determination was performed on Thermo Scientific Syncronis C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution（pH adjusted to 3.0 with triethylamine）（30 ∶ 70，V/V） at the flow rate of 0.3 mL/min. The detection wavelength was set at 345 nm， and column temperature was 25 ℃. The sample size was 2 μL. RESULTS： The linear ranges were 4.08-163 ng for epiberberine（r＝0.999 9）， 5.72-229 ng for jateorhizine hydrochloride （r＝0.999 8）， 5.67-227 ng for coptisine（r＝0.999 9）， 6.78-271 ng for palmatine hydrochloride（r＝0.999 6），13.2-526 ng for berberine hydrochloride（r＝0.999 9）. RSDs of precision， stability and reproducibility tests were lower than 2.0％. The recoveries were 96.83％-103.92％（RSD＝2.2％，n＝9）， 97.62％-104.85％（RSD＝2.9％，n＝9），99.44％-104.18％（RSD＝1.8％，n＝9）， 98.64％-104.42％（RSD＝1.8％，n＝9），98.24％-102.40％（RSD＝1.5％，n＝9），respectively. CONCLUSIONS： The developed method is simple， sensitive， rapid and accurate. It is suitable for simultaneous determination of 5 coptis alkaloids in Lianshen tonglin tablets.