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目的:考察维药毛菊苣95%乙醇提取物(CG-I)对小鼠免疫性肝损伤的保护作用及机制,为后期筛选其有效部位提供参考。方法:将60只小鼠随机分为空白对照组(生理盐水)、模型组(生理盐水)、阳性对照组(甘草酸二铵,100 mg/kg)和CG-I高、中、低剂量组(以生药量计分别为200、100、50 g/kg),每组10只,每天ig给药1次,连续给药10 d。末次给药后1 h,除空白对照组外的其余各组小鼠均通过尾iv刀豆蛋白A(Con-A)诱导免疫性肝损伤。造模8 h后,检测小鼠血清中肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)和白细胞介素1β(IL-1β)含量,计算肝、脾指数,观察肝组织病理学变化并检测肝组织中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、谷胱甘肽S-转移酶(GST)、碱性磷酸酶(AKP)、总超氧化物歧化酶(T-SOD)、丙二醛(MDA)水平。结果:与空白对照组比较,模型组小鼠血清中TNF-α、IFN-γ、IL-1β含量显著增加,肝、脾指数及肝组织中AST、ALT、GST、AKP、T-SOD水平显著升高,肝组织中MDA水平显著降低,差异均有统计学意义(P<0.05或P<0.01);小鼠肝索排列杂乱,肝细胞出现肿胀、坏死等病变。与模型组比较,除CG-I低剂量组小鼠肝组织中AST、ALT、AKP水平以及CG-I中、低剂量组小鼠血清中MDA、IFN-γ含量降低不显著外,其余各指标均显著改善(P<0.05或P<0.01);肝组织病理变化均不同程度地减轻。结论:CG-I对Con-A所致小鼠免疫性肝损伤具有保护作用,尤其以高、中剂量效果较好;其机制可能与抗氧化、抗炎作用有关。
OBJECTIVE: To investigate the protective effect and mechanism of Wei medicine Cichorium glandulosum 95% ethanol extract (CG-I) on immunological liver injury in mice, and provide reference for post-screening its effective site. METHODS: 60 mice were randomly divided into blank control group (normal saline), model group (normal saline), positive control group (Diammonium glycyrrhizinate, 100 mg/kg) and CG-I high-dose, medium-dose,low-dose groups (calculated by crude drugs as 200,100,50 g/kg), 10 in each group, intragastrically administrated once every day, for 10 d. After 1 h of last administration, except for blank control group, mice in other groups were intravenously injected Con-A in tail to induce immunological liver injury. After 8 h of modeling,tumor necrosis factor α (TNF-α), interferon γ (IFN-γ), interleukin 1β (IL-1β) contents in serum were detected; liver and spleen indexes were calculated. The pathological changes in liver tissue were observed, and aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione S-transferase (GST), alkaline phosphatase (AKP), total superoxide dismutase (T-SOD), malondialdehyde (MDA) levels in liver tissue were detected. RESULTS: Compared with blank control group, TNF-α, IFN-γ, IL-1β contents in serum in model group were significantly increased; liver, spleen indexes and AST, ALT, GST, AKP, T-SOD levels in liver tissue were significantly increased; and MDA level in liver tissue was significantly reduced, with statistical significances (P<0.05 or P<0.01);liver of mice in model group was cluttered, showing swelling, necrosis and other diseases in liver cells. Compared with model group, except that AST, ALT, AKP levels in liver tissue in CG-I low-dose group and MDA, IFN-γ contents in serum in CG-I medium-dose, low-dose groups had no significant decrease, other indexes were significantly improved (P<0.05 or P<0.01);and pathological changes in liver tissue were relieved to varying degrees.CONCLUSIONS:CG-I shows protective effect on Con-A-induced immunological liver injury in mice, especially the high dose and medium dose. The mechanism may be associated with its anti-oxidation and anti-inflammatory effects.
维药毛菊苣95%乙醇提取物免疫性肝损伤抗氧化抗炎小鼠
Wei medicineCichorium glandulosum95% ethanol extractImmunological liver injuryAnti-oxidantanti-inflammatoryMice
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