OBJECTIVE： To study the potential targets of Guizhi decoction regulating Ying and Wei based on “correspondence between formulation and syndrome” relationship and reverse molecular docking technology. METHODS： Active ingredients related to Guizhi decoction were obtained by literature retrievals， and PharmMapper Server was used for reverse molecular docking for active ingredients. The potential receptors of Guizhi decoction were found on the basis of docking scores， then AutoDock Vina was conducted for positive molecular docking test to observe the affinity between the ligand and the receptor molecule. 75 rats were randomly divided into normal group， model group， Guizhi decoction high-dose， medium-dose， low-dose groups （12， 8， 4 g/kg）， 15 in each group. Except for normal group， rats in other groups were induced for models with disharmony between Ying and Wei. After modeling， rats were intragastrically administrated once a day， for 5 d. After administration， 11β-HSD1 expression levels in adipose tissue and muscle tissue of rats were detected. RESULTS： Reverse molecular docking for active ingredients of Guizhi decoction found that 11β-HSD1 was the frequent receptor， and positive docking test confirmed that uralsaponin b， glycyrrhetnic acid and carbenoxolone and so on had higher affinity with 11β-HSD1. Results of animal test showed， compared with normal group， 11β- HSD1 expression levels in adipose tissue and muscle tissue in model group were increased （P＜0.05）； and compared with model group， 11β-HSD1 expression levels in adipose tissue and muscle tissue in Guizhi decoction high-dose group were decreased （P＜0.05）. CONCLUSIONS： Guizhi decoction regulating Ying and Wei has certain correlation with 11β-HSD1， while the reverse molecular docking technology can provide a feasible technical way to study “correspondence between formulation and syndrome” relationship.