OBJECTIVE： To establish the method for simultaneous determination of the content of metronidazole， clindamycin hydrochloride， spironolactone and vitamin B6 in Compound metronidazole clindamycin cream. METHODS： HPLC method was adopted. The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-[0.68％ dipotassium hydrogen phosphate solution （containing 1％ triethylamine）] （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 210 nm， and column temperature was 25 ℃. The sample size was 20 μL. RESULTS： The linear ranges of metronidazole， clindamycin hydrochloride， spironolactone and vitamin B6 were respectively 20.0-120.0 μg/mL（r＝0.999 9），10.0-60.0 μg/mL（r＝0.999 1）， 4.0-24.0 μg/mL（r＝0.999 4），10.0-60.0 μg/mL （r＝0.999 4）. The quantitative limits were respectively 13.06， 7.36， 2.43， 6.42 μg/mL. The detection limits were 4.18， 1.82， 0.76， 1.79 μg/mL. RSDs of the precision， stability， repeatability tests were all less than 2％. The recovery rates were respectively 98.97％-100.91％（RSD＝0.79％，n＝9）， 99.02％-101.80％（RSD＝1.03％，n＝9）， 98.18％-101.05％ （RSD＝1.01％，n＝9）and 98.75％-101.36％（RSD＝0.87％，n＝9）.  CONCLUSIONS： The method is simple， rapid， accurate and can be used for the determination of metronidazole， clindamycin hydrochloride， spironolactone and vitamin B6 in Compound clindamycin metronidazole cream.