OBJECTIVE： To study the chemical constituents of the ethyl acetate extraction portion of Polygonum bistorta， provide scientific basis for the basic research of its effective substances. METHODS： Taking the ethyl acetate extraction portion of P. bistorta 95％ ethanol extract， positive phase and reversed phase column chromatography were used for repeated isolation and purification， and the structures of the purified compounds were identified on the basis of physicochemical properties or spectral data. RESULTS： 11 monomer compounds were isolated and identified as β-sitosterol （1）， dihydromyricetin （2）， quercetin （3）， kaempferol （4）， epicatechin （5）， chlorogenic acid （6）， rutin （7）， gallic acid （8）， 1，2，3，4-tetrahydro-8-hydroxy-4-isopropyl-1-methylnaphthalene-6-carboxylic acid （9）， protocatechuic acid （10） and ellagic acid （11）. CONCLUSIONS： The literature is confirmed that compound 2 is isolated from P. bistorta for the first time and compound 9 is isolated from Polygonum for the first time. The related research results have provided experimental references for further clarifying the effective substances of P. bistorta.