OBJECTIVE： To provide reference for rational use of antibiotics in the clinic. METHODS： Blood culture positive specimens were collected from our hospital during Jan. 2011-Dec.2016. Distribution of bloodstream infection （BSI） pathogens and drug resistance were analyzed in our hospital retrospectively. RESULTS： During 2011-2016， 26 034 blood culture specimens isolated from inpatients of our hospital were examined， including 1 775 positive specimens with positive rate of 6.82％. The specimens mainly came from tumor hematology department （10.65％）， neurosurgery department （8.28％） and pediatric department （8.00％）. A total of 1 775 strains of pathogens were detected， including 967 strains of Gram-negative bacteria （54.48％） mainly as Escherichia coli， Klebsiella pneumoniae， 649 strains of Gram-positive bacteria （36.56％） mainly as Coagulase negative Staphylococci， Staphylococcus aureus and 159 strains of fungus （8.96％） mainly as Candida albicans. E. coli and K. pneumoniae were resistant to common antibiotics to different extents， but sensitive to piperacillin sodium and tazobactam sodium， imipenem， meropenem. Acinetobacter baumanii was highly resistant to enzyme inhibitors， cephalosporins， aminoglycosides， quinolones. Pseudomonas aeruginosa was sensitive to third-generation cephalosporins， aminoglycosides and quinolones. S. aureus was highly resistant to penicillins， cephalosporins and aminoglycosides. Resistance rate of Coagulase negative Staphylococci to most commonly used antibiotics was higher than 40％. Above two bacteria were sensitive to linezolid and vancomycin with resistance rate of 0. A total of 205 strains of ESBLs-producing E. coli （42.01％）， 64 strains of ESBLs-producing K. pneumoniae （30.33％） and 31 strains of Methicillin-resistant S. aureus （17.61％） were detected. No vancomycin-resistant Enterococcus or vancomycin-resistant S. aureus was detected. CONCLUSIONS： BSI pathogens mainly distribute in tumor hematology department of our hospital. BSI pathogens mainly include Enterobacteriaceae and Staphylococcus， and also involve fungus. The situation of drug resistance and enzyme production are not optimistic. Antibiotics， which are sensitive to the major pathogens， include carbapenems， linezolid and vancomycin.