OBJECTIVE： To study the chemical constituents of Zingiberis Rhizoma. METHODS： The compounds of Zingiberis Rhizoma were classified and purified by silica gel， Sephadex LH-20 column chromatography and thin layer chromatography （TLC）. The structure of compounds were analyzed and identified according to chemical property and spectrum data. RESULTS & CONCLUSIONS： Five compounds were isolated from Zingiberis Rhizoma， i.e. methyl-6- gingerol （1）， 4- gingerol （2）， β-eudesmol （3）， 2， 5-dihydroxybisabola-3，10-diene （4）， 6-shogaol （5）. The compounds 2， 3， 4 are isolated from Zingiberis Rhizoma for the first time.