OBJECTIVE： To prepare the vinorelbine-tetrandrine liposomes modified with RGD， and study the inhibitory effect on glioma C6 cells. METHODS： Film dispersion method and ammonium sulfate gradient method were used to prepare the vinorelbine-tetrandrine liposomes modified with RGD， and the morphology and particle size distribution were observed. The vinorelbine content was determined， and sulforhodamine B method was used to respectively determine the inhibitory effects of blank targeting liposomes， normal vinorelbine liposomes and vinorelbine-tetrandrine liposomes modified with RGD on C6 cells. RESULTS： The prepared vinorelbine-tetrandrine liposomes modified with RGD were spherical or almost spherical with smooth surface， and particle size was about 120 nm. The average content of vinorelbine was 28.27 μg/mL （RSD＝0.38％， n＝3）. Blank targeting liposomes had no significant effect on the growth of C6 cells； vinorelbine-tetrandrine liposomes modified with RGD can obviously inhibit the growth of C6 cells， and cell viability after its effect was significantly lower than normal vinorelbine liposomes （P＜0.05）. CONCLUSIONS： Vinorelbine-tetrandrine liposomes modified with RGD are successfully prepared， and they show obvious inhibitory effects on the growth of C6 cells.