OBJECTIVE： To study the extraction rate and speciation of antivitiligo-related elements in Opercalina turperthum by water extraction and semi-bionic extraction. METHODS： Water extraction and semi-bionic extraction were respectively used. Water-soluble state and suspension state in extract of O. turperthum were separated by microporous filtering film； organic and inorganic trace elements in water-soluble state were separated by macroporous resin. The contents of Cu， Zn， Fe， Ca， Mg， Mn and Sr were detected by flame atomic adsorption spectrophotometry， and speciation analysis was conducted. RESULTS： After water extraction， extraction rates of 7 trace elements were 40.47％-72.49％； ratio of suspended particles was 3.69％-8.78％； ratio of organic state/inorganic state was 104.36％ in water-soluble state of Sr and 3.94％-48.39％ in water-soluble state of Cu， Zn， Fe， Ca， Mg， Mn. After semi-bionic extraction， except for Mn， extraction rates of trace elements were higher than water extraction， extraction rates of Cu， Zn， Fe， Ca， Mg， Sr were 77.69％-90.19％； ratio of suspended particles was 0.39％-8.57％； the ratio of organic state/inorganic state was 72.74％-180.79％ in water-soluble state of elements. CONCLUSIONS： Cu， Zn， Fe， Ca， Mg， Mn and Sr in O. turperthum are dissolved easily， mainly existing in the form of inorganic state. After semi-bionic extraction， except for Mn， the dissolution rate of other elements and the proportion of organic trace elements after dissolution increase significantly. Both dissolution and effective utilization rate of trace elements by semi-bionic extraction are higher than water extraction.