OBJECTIVE： To establish HPLC fingerprint for the leaves of Camptotheca acuminante in Guizhou. METHODS： HPLC method was performed. The determination was performed on Gemini-NX C18 column with mobile phase consisted of acetonitrile-0.2％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 370 nm， and the column temperature maintained at 30 ℃. The sample size was 10 μL. Using sorbitol as a reference， HPLC fingerprints of 14 batches of the leaves of C. acuminante were determined. The chromatographic fingerprint was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004 A） in terms of common peak indentification， similarity evaluation and cluster analysis. RESULTS： There were 10 common peaks in HPLC fingerprints for 14 batches of the leaves of C. acuminate. And the similarity of 13 batches of the leaves of C. acuminate was greater than 0.90， and that of another one was less than 0.90. The leaves of C. acuminate were classified into 3 groups. CONCLUSIONS： The established fingerprint can provide reference for identification and quality evaluation of the leaves of C. acuminate.