OBJECTIVE： To establish a method for simultaneous determination of paeoniflorin， naringin， neohesperidin， notoginsenoside R1， ginsenoside Rg1 and ginsenoside Rb1 in Quyu zhitong mixture. METHODS： HPLC method was adopted. The determination was performed on Waters Xbridge C18 column with mobile phase consisted of 0.1％ phosphoric acid-acetonitrile （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 203 nm， and column temperature was 20 ℃. The sample size was 5 μL. RESULTS： The linear ranges of paeoniflorin， naringin， neohesperidin， notoginsenoside R1， ginsenoside Rg1 and ginsenoside Rb1 were 0.273 4-2.734 μg（r＝0.999 9），0.119 1-1.191 μg（r＝0.999 9），0.081 5-0.815 μg（r＝0.999 9），0.622 8-6.228 μg（r＝0.999 9），0.807 2-8.072 μg（r＝0.999 9），1.036 4-10.364 μg（r＝0.999 9）. The limits of quantitation were 0.082 0， 0.029 8， 0.028 5， 0.436 0， 0.403 6， 0.310 9 μg. The limits of detection were 0.027 9， 0.009 5， 0.010 2， 0.124 6， 0.121 1，0.093 3 μg. RSDs of precision， stability and reproducibility tests were lower than 2.0％. Recoveries were 97.85％-100.34％（RSD＝0.81％，n＝6），98.14％-101.22％（RSD＝1.09％，n＝6），98.42％-102.15％（RSD＝1.29％，n＝6）， 97.77％-100.25％（RSD＝0.96％，n＝6），97.32％-99.53％（RSD＝0.81％，n＝6） and 98.28％-101.51％ （RSD＝1.11％，n＝6）. CONCLUSIONS： The method is simple， rapid， stable， feasible and reproducible， and can be used for the simultaneous determination 6 components in Quyu zhitong mixture.