OBJECTIVE： To explore basic technology for synthesis of active ingredients of Ophiocordyceps xuefengensis， and provide necessary technical support for comprehensive development of O. xuefengensis sourse. METHODS： Submerged fermentation method was used to cultivate the mycelium， achieving efficient synthesis of active ingredients by controlling medium composition and cultivation conditions. Using the bacteria as starting strain， the effects of different carbon sources （sucrose， glucose and soluble starch）， different nitrogen sources （peptone， yeast extract powder， yeast extract， sodium nitrate， potassium nitrate and urea）， different vitamin B （vitamin B1 and vitamin B complex） and different initial pH （pH was set at 4， 5， 6， 7， 8 and 9， respectively） on mycelial growth， extracellular and intracellular polysaccharide synthesis， cordycepin synthesis and intracellular triterpenoid synthesis were investigated to screen the optimal medium composition. RESULTS： The optimal carbon source， nitrogen source， vitamin B and initial pH were sucrose， yeast extract powder， vitamin B1 and 8， respectively. High biomass and metabolite accumulation levels can be obtained when carbon source was sucrose， nitrogen source was yeast extract powder， adding 0.1 g/L vitamin B1 with initial pH of 8. CONCLUSIONS： O. xuefengensis can efficiently accumulate metabolites， and achieve the optimization of strain cell growth and synthesis of active metabolite by optimizing and controlling the fermentation process.