OBJECTIVE： To establish a method for the determination of related substances in Vildagliptin tablets. METHODS： HPLC method was adopted. The determination was performed on Xterra MS C18 column with mobile phase consisted of [phosphate buffer-water-acetonitrie-methanol （400 ∶ 600 ∶ 15 ∶ 15，V/V/V/V）]-[phosphate buffer-acetonitrile-methanol （400 ∶ 450 ∶ 150，V/V/V）] （gradient elution） at the flow rate of 1.2 mL/min. The detection wavelength was set at 210 nm and column temperature was 35 ℃. The sample size was 10 μL. RESULTS： The linear ranges were 18.80-188.0 μg/mL for impurity A （r＝0.999 5）， 22.64-226.4 μg/mL for impurity B （r＝0.999 6）， 21.74-217.4 μg/mL for impurity C （r＝0.999 7）， 19.12-191.2 μg/mL for impurity D （r＝0.999 8）. The limits of detection were 4.18， 2.68， 1.12， 1.34 μg/mL， respectively； RSDs of precision， stability and reproducibility tests were lower than 3％； the recoveries of impurity A， B， C and D were 97.9％-103.1％（RSD＝2.01％，n＝9）， 98.8％-104.1％（RSD＝1.93％， n＝9）， 98.0％-103.6％（RSD＝1.81％，n＝9）， 100.8％-104.1％（RSD＝0.98％，n＝9），respectively. CONCLUSIONS： The method is simple and accurate， and can be used for the determination of related substances in Vildagliptin tablets.