OBJECTIVE： To improve HPLC for content determination of ketoprofen in Ketoprofen enteric-coated capsules. METHODS： HPLC method was adopted. The determination was performed on Chiralpak IC column with mobile phase consisted of n-hexane  （0.1％ TFA）-isopropanol （90 ∶ 10，V/V） at a flow rate of 0.8 mL/min. The detection wavelength was set at 268 nm， and column temperature was 25 ℃. The sample size was 10 μL. RESULTS： The linear range of ketoprofen were 0.025-0.5 mg/mL（r＝0.999 8）. The limit of quantitation was 1.0 mg/L， and limit of detection was 0.2 mg/L. RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 96.36％-100.32％ （RSD＝1.87％，n＝6）. CONCLUSIONS： The method is simple， accurate and rapid， and can be used for the content determination of ketoprofen in Ketoprofen enteric-coated capsules.