OBJECTIVE： To screen the method for extracting total flavonoids in persimmon leaves and optimize extraction technology. METHODS： Using extract quality and flavonoids content as indexes， the effects of extracting total flavonoids in persimmon leaves by ethanol refluxing method， enzyme method （cellulase， β-glucanase and complex enzyme mixed by equal amounts of both）， semi-bionic method and semi-bionic-enzyme method （the same enzymes） were compared. Using flavonoids content as index， solid-liquid radio， reflux temperature， reflux time as factors， orthogonal test was designed to optimize the extraction technology conditions of flavonoids in persimmon leaves by semi-bionic-enzyme method， and the verification test was conducted. RESULTS： The extract quantity and flavonoids content by semi-bionic-enzyme method was the highest among the 4 extraction methods， and the complex enzyme was the most suitable； the optimized extracting condition of semi-bionic-enzyme method were as follows as solid-liquid radio of 1 ∶ 14， reflux temperature of 50 ℃， reflux time of 2.0 h； extraction rates of flavonoids in 3 verification tests were 5.9％， 5.8％， 5.9％ （RSD＝0.98％， n＝3）. CONCLUSIONS： The optimized semi-bionic-enzyme method is efficient and stable in extracting flavonoids in persimmon leaves.