OBJECTIVE： To compare the differences between artificially cultivated and wild Xinjiang Artemisia rupestris， and screen the different components. METHODS： HPLC-MS was adopted to establish the fingerprints of artificially cultivated and wild Xinjiang A. rupestris from different origin and harvest time. Principal component analysis was conducted by Marker ViewTM software and SIMCA-P 11.5 software， the characteristics of principal components were analyzed， difference variable was screened， and different components of artificially cultivated and wild varieties were obtained. RESULTS： Fingerprints of 22 batches of A. rupestris （12 batches of wild varieties， 10 batches of artificially cultivated varieties） were established. According to the principal component analysis， artificially cultivated and wild varieties were well grouped， with obvious differences； the principal components of artificially cultivated varieties with different harvest time showed certain difference， mainly before and after flowering， concentrating in to-be flowering and full flowering periods. Wild varieties from different origins had obvious regional difference， showing certain differences in composition and content. 268 variables were found in matrix of positive ion mode and 155 in negative ion mode. 28 groups of variables were extracted by difference variable， and 19 variables were determined. CONCLUSIONS： Artificially cultivated and wild varieties have obvious difference in principal component， mainly in flowering period and picking places. It can provide theoretical basis for the standardized cultivation and origin protection of Xinjiang A. rupestris.