OBJECTIVE： To study the effect and its mechanism of Fuzheng Jiedu Quyu formula （short for “Fuzheng formula”） combined with oxaliplatin （L-OHP） on human colon cancer HT-29 cell proliferation and apoptosis. METHODS： HT-29 cells were divided into blank control group （without drugs）， Fuzheng formula group （1 000 mg/L）， L-OHP group （31.25 mg/L） and combination group （1 000 mg/L Fuzheng formula+31.25 mg/L L-OHP）. After cultured with corresponding drug for 48 h， MTT method was used to detect the cell proliferation； the changes of cellular morphology were observed by invert microscope； flow cytometry was used to detect the cell cycle and apoptosis rate. Proapoptotic gene Bax， apoptotic gene Bcl-2 mRNA expressions were determined by real-time fluorescence quantitative polymerase chain reaction method； Bax， Bcl-2 protein expressions were assayed by Western blot. RESULTS： Compared with blank control group， cell proliferation was inhibited in L-OHP group and combination group； cell proportion was increased in S stage， G2/M stage and decreased in G0/G1 stage （P＜0.05）. Cell apoptosis rate in L-OHP group， Fuzheng formula group and combination group was increased； Bax mRNA and protein expression were up-regulated， Bcl-2 mRNA expression was downregulated （P＜0.05）； and combination group changed more obviously than the single drug groups （P＜0.05）. CONCLUSIONS： Fuzheng formula combined with L-OHP can inhibit HT-29 cell proliferation and promote its apoptosis， showing better effects than either of the two drugs alone. The mechanism may be associated with up-regulation of Bax gene and protein expressions and down-regulation of Bcl-2 gene expressions in cells.