OBJECTIVE： To screen the α-glucosidase inhibitory active part from Pothos chinensis. METHODS： The aqueous extractions of P. chinensis were extracted by petroleum， ethyl acetate， n-butyl alcohol in turn to obtain different polarparts. Effect of each part on α-glucosidase inhibitory activity was determined， and enzyme inhibition kinetics was conducted for the screened parts with strong activity and relatively high yield rate； effects of each part on blood glucose level of mice loaded with glucose， sucrose and starch were respectively determined （using Acacoside tablet as positive control）. RESULTS： Enzyme inhibition kinetics in vitro showed the ethyl acetate part [yield rate was 0.40％， enzyme activity inhibition rate was （72.90±2.85）％] had strong α-glucosidase inhibitory activity and showed a dose-dependent， fast， non-competitive and reversible model. Results of in vivo glucose tolerance indicated that Acacoside tablet and each part of P. chinensis had no effects on blood glucose level of glucose-loaded mice （P＞0.05）； while Acacoside tablet and ethyl acetate part in P. chinensis could reduce 30， 60 min blood glucose level of sucrose-loaded mice and 30， 60， 120 min blood glucose level of starch-loaded mice （P＜0.05 or P＜0.01）. CONCLUSIONS： Ethyl acetate part is the α-glucosidase inhibitory active part from Yao medicine P. chinensis.