OBJECTIVE： To establish a method for the content determination of matrine， sophocarpine， emodin， rhein， chlorogenic acid， sarkosaponin a， aloe-emodin in Kuhuang injection.METHODS： UPLC-MS/MS was adopted. The determination was performed on Phenomenex Kenetix C18 column with mobile phase consisted of methanol-0.1％ formic acid （gradient elution） at the flow rate of 0.5 mL/min. The column temperature was set at 20 ℃， and injector temperature was set at 10 ℃. The equilibrium time was 4 min and sample size was 5 μL. Ionization mode was electrospray ionization with spray voltage of 5 500 V and －4 500 V， atomizing air pressure of 4.14×105 Pa， heater pressure of 4.48×105 Pa， curtain air of 1.72×105 Pa and ion source temperature of 600 ℃. The work mode was multiple reaction monitoring mode. RESULTS： The linear range were 1.25-80.0 ng/mL for matrine （r＝0.999 3），1.10-70.0 ng/mL for sophocarpine （r＝0.999 5）， 0.16-10.5 ng/mL for emodin （r＝0.999 3）， 2.61-168 ng/mL for rhein （r＝0.999 3）， 1.50-96.0 ng/mL for chlorogenic acid （r＝0.999 3），1.48-94.5 ng/mL for sarkosaponin a （r＝0.999 6） and 6.11-391 ng/mL for aloe-emodin （r＝0.999 1）. The limits of quantification were 0.061，0.109，0.041，1.313，0.500，0.492，3.055 ng/mL，limits of detection were 0.025，0.054，0.016，0.656，0.150，0.148，1.528 ng/mL. RSDs of precision， stability and reproducibility tests were all no more than 3％. The recoveries of them were 95.45％-99.45％（RSD＝1.43％，n＝6）， 97.50％-101.00％（RSD＝1.50％，n＝6）， 95.67％-101.73％（RSD＝2.85％，n＝6）， 97.17％-100.57％ （RSD＝1.16％，n＝6），95.19％-98.90％（RSD＝1.71％，n＝6）， 95.38％-103.85％（RSD＝3.39％，n＝6）， 95.50％-101.17％（RSD＝1.20％，n＝6）， respectively. CONCLUSIONS： The method is simple， effective， accurate， reliable and suitable for simultaneous determination for 7 components in Kuhuang injection.