OBJECTIVE： To establish a method for the determination of donkey-hide gelatin in Lüjiao buxue granules. METHODS： Trypsin was used for enzymatic hydrolysis of donkey-hide gelatin. UPLC-QQQ/MS was adopted for the determination of donkey-hide gelatin in the preparation： Hypersil GOLD C18 column was used with mobile phase consisted of 0.1％ formic acid solution-acetonitrile （gradient elution） at the flow rate of 0.3 mL/min. The column temperature was 30 ℃， and sample size was 5 μL. Mass chromatography condition was that ion source was electrospray ion source； ionization mode was ESI+； multiple response monitoring was adopted， and characteristic molecular ion peaks of donkey-hide gelatin with mass-to-charge ratio of m/z 539.8 （double charge） → 612.4 and m/z 539.8 （double charge） → 923.8 were used as detection ion pair. Spray voltage was 3 100 V； sheath gas was 20 Arb； auxiliary gas was 8 Arb； ion transfer tube temperature was 350 ℃； evaporation temperature was 350 ℃； scan mode was SRM. Collision gas was high purity argon. RESULTS： The sample size of donkey-hide gelatin ranged 20.24-2 024 μg/mL（r＝0.997 8）. The detection limit was 1％. RSDs of precision， stability and reproducibility tests were all lower than 6.0％. The characteristic molecular ion peaks of donkey-hide gelatin could be detected in 27 batches of samples. CONCLUSIONS： The method is specific and can be used for the determination of donkey-hide gelatin in Lüjiao buxue granules.