OBJECTIVE： To establish a method for the determination of related substances in Pantoprazole sodium for injections. METHODS： HPLC method was adopted. The determination was performed on Kromasil Hypersil ODS column with mobile phases consisting of 0.01 mol/L potassium dihydrogen phosphate buffer solution （pH adjusted to 7.0）-acetonitrile （gradient elution） at a flow rate of 1.0 mL/min. The detection wavelength was set at 290 nm， and the column temperature was 40 ℃，and injection volume was 20 μL. RESULTS： The linear ranges of impurity A， impurity B， impurity C+E， and impurity D were 0.416 8-1.042 0 μg/mL（r＝0.999 8），0.195 0-0.487 5 μg/mL（r＝0.999 9），0.389 0-0.972 5 μg/mL（r＝0.999 8），0.198 6-0.496 5 μg/mL（r＝0.999 8）， respectively. The limits of quantitation were 0.834， 0.780， 1.556， 0.794 ng/mL； the limits of detection were 0.417， 0.390， 0.778， 0.397 ng/mL， respectively. RSD of precision test was lower than 1.0％； in repetitive test， RSD for total peak area of impurity was lower than 1.0％； the recoveries were 98.81％-102.49％（RSD＝1.18％，n＝9），95.31％-98.44％（RSD＝0.91％，n＝9）， 96.88％-98.44％（RSD＝0.52％，n＝9） and 97.87％-101.28％（RSD＝1.05％，n＝9）. CONCLUSIONS： The method is convenient， accurate and suitable for the determination of related substance in Pantoprazole sodium for injection.