OBJECTIVE： To optimize ultrasonic extraction technology for polysaccharide from Aconiti lateralis radix praeparata （ALRP）. METHODS： Uniform design method was applied to investigate the effects of liquid material ratio， ultrasonic time and ultrasonic temperature on extraction rate of polysaccharide from ALRP. Verification test was conducted and compared with the results of conventional decoction and boiling method. RESULTS： Optimized ultrasonic extraction technology was as follow as liquid material ratio of 10 mL/g， ultrasonic time of 34 min and ultrasonic temperature of 73 ℃. The polysaccharide extraction rate in verification test was 19.05％ （RSD＝0.60％， n＝3）， relative error of the predicted value （19.44％） was 2.0％， while the extraction rate was higher than decoction and boiling method （16.42％）. CONCLUSIONS： Optimized ultrasonic extraction technology is simple， rapid and stable with high extraction rate， which is suitable for extracting polysaccharide from ALRP.