OBJECTIVE： To establish a method for simultaneous determination of chlorogenic acid， geniposide， gentiopicroside， baicalin， berberine hydrochloride and chrysophanol in Longze xiongdan capsule. METHODS： HPLC method was adopted. The separation was performed on Dikma Platisil ODS column with mobile phase consisting of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength were set at 254 nm for geniposide， gentiopicroside， baicalin， berberine hydrochloride and chrysophanol and 327 nm for chlorogenic acid. The column temperature was 30 ℃. RESUITS： Linear ranges of chlorogenic acid， geniposide， gentiopicroside， baicalin， berberine hydrochloride and chrysophanol were 0.008 2-0.164 μg （r＝0.999 8），0.027-0.540 μg（r＝0.999 7），0.025 25-0.505 μg（r＝0.999 8），0.046-0.920 μg（r＝0.999 7），0.059-1.180 μg（r＝0.999 5），0.008 05-0.161 μg（r＝0.999 4）， respectively； the limits of quantitation were 0.61， 0.59， 0.87， 0.51， 0.92， 0.65 ng， and the limits of detection were 1.67， 1.73， 1.96， 1.82， 2.31， 1.87 ng. RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 95.34％-98.27％（RSD＝1.2％，n＝9）， 98.18％-101.92％（RSD＝1.4％，n＝9）， 97.36％-101.09％（RSD＝1.0％，n＝9），95.24％-98.93％（RSD＝1.3％，n＝9）， 95.76％-99.34％（RSD＝1.5％，n＝9）， 95.00％-98.75％（RSD＝1.4％，n＝9）， respectively. CONCLUSIONS： The method is simple， accurate， reproducible and suitable for simultaneous determination of chlorogenic acid， geniposide， gentiopicroside， baicalin， berberine hydrochloride， chrysophanol in Longze xiongdan capsule.