OBJECTIVE： To establish a method of contents determination of 17 amino acids in Fritillariae Pallidiflorae Bulbus produced in Xinjiang. METHODS： Cation-exchange column was used to separate 17 kinds of amino acids； post-column derivatization liquid chromatography was used to determine the contents of amino acids： the column was strong sulfonic acid cation exchange resin LCAK06/Na with mobile phase  A （weighing trisodium citrate 11.9 g， citric acid 6 g， phenol 1 g， dissolving by water， then adding 65 mL of methanol and 6 mL of concentrated hydrochloric acid， bringing the volume with tap water， adjusting pH to 3.4）， mobile phase B （weighing trisodium citrate 11.9 g， NaOH 2.8 g， phenol 1 g， boric acid 5.0 g， adding water to dissolve， adjusting pH to 10.8）， gradient elution，flow rate was 0.45 mL/min for A pump and 0.25 mL/min for M pump，the detection wavelength was 440 nm for proline and 570 nm for the remaining amino acids，the injection volume was 50 μL. RESULTS： The linear range were 20～400 nmol/mL of aspartic acid，threonine， serine， glutamic acid， glycine， alanine， valine， methionine， isoleucine， leucine， tyrosine， phenylalanine， histidine， lysine， arginine， proline，10-200 nmol/mL of cystine（r were higher than 0.989 0）； RSDs of precision， stability and reproducibility tests were lower than 2.0％； limits of detection were 0.16 nmol/mL except for cystine（0.08 nmol/mL）； recovery was 98.5％-99.5％（RSD was 0.06％-0.21％，n＝6）. CONCLUSIONS： The method is simple with good precision， stability and reproducibility， and can be used for the simultaneous determination of amino acids in Fritillariae Pallidiflorae Bulbus produced in Xinjiang.